Defective processing and trafficking of water channels in nephrogenic diabetes insipidus.

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Title: Defective processing and trafficking of water channels in nephrogenic diabetes insipidus.
Authors: Kamsteeg, Erik-Jan; Deen, Peter M.T.; van Os, Carel
Publisher: Experimental Nephrology
Date Published: November 01, 2000
Reference Number: 548
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Nephrogenic diabetes insipidus (NDI) is a disease characterized by the inability of the kidney to concentrate urine upon stimulation with vasopressin. Mutations in the gene for aquaporin-2 (AQP2) are the cause of the autosomal recessive and autosomal dominant forms of NDI. Mutant AQP2 proteins, found in autosomal recessive NDI, were shown to be misfolded and retarded in the endoplasmic reticulum. One mutant protein leading to autosomal dominant NDI, E258K, has been analyzed in detail. It was shown that this mutant was not retarded in the endoplasmic reticulum but mainly retained in the Golgi network. Furthermore, this particular mutant was able to form heterotetramers with wild-type AQP2, in contrast to mutants found in autosomal recessive NDI. The subsequent misrouting of complexes containing wild-type and mutant AQP2 proteins explains dominant NDI. Copyright 2000 S. Karger AG, Basel