Functional Involvement of VAMP/Synaptobrevin-2 in cAMP-Stimulated Aquaporin 2 Translocation in Renal Collecting Duct Cells
|Title:||Functional Involvement of VAMP/Synaptobrevin-2 in cAMP-Stimulated Aquaporin 2 Translocation in Renal Collecting Duct Cells|
|Authors:||Gouraud, Sabine; Laera, Antonia; Calamita, Giuseppe; Carmosino, Monica; Procino, Giuseppe; Rossetto, Ornella; Mannucci, Roberta; Rosenthal, Walter; Svelto, Maria; Valenti, Giovanna|
|Publisher:||Journal of Cell Science|
|Date Published:||September 15, 2002|
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This translation by the NDI Foundation is to assist the lay reader. To provide a clear, accessible interpretation of the original article, we eliminated or simplified some technical detail and complicated scientific language. We concentrated our translation on those aspects of the article dealing directly with NDI. The NDI Foundation thanks the researchers for their work toward understanding and more effectively treating this disorder.
© Copyright NDI Foundation 2007 (JC)
The AQP2 containing vesicles cannot make this trip to the membrane by themselves. They are helped by other proteins, not all of which have been determined. Gouraud, et al., suspected that a type of soluble N-ethymaleimide sensitive factor-attachment protein receptor (SNARE), namely VAMP-2, was involved in the AQP2 vesicle movement to the cell membrane.
To test their hypothesis, they generated laboratory cell cultures that contained AQP2s and VAMP-2s. When stimulated with cAMP, the AQP2s made their journey to the membrane. The researchers then added tetanus neurotoxins (TeNTs) to these cultures. TeNT is known to split VAMP-2 and thus interfere with its ability to function. After TeNT was injected into the cell cultures, they were again stimulated with cAMP. The result was that there was no travel of AQP2 laden vesicles to the cell membrane. This result provides evidence that VAMP-2 is directly involved in the cAMP-2 stimulated movement of AQP2 from the cell interior to cell membrane.