In Vitro Micro-Autoradiography of Atrial Natriuretic Peptide in Biopsy Specimens from Patients with Renal Diseases
|Title:||In Vitro Micro-Autoradiography of Atrial Natriuretic Peptide in Biopsy Specimens from Patients with Renal Diseases|
|Authors:||Ogura, Toshio; Asano, Naoko; Katayama, Eriko; Oishi, Tetsuya; Mimura, Yukari; Hironaka, MD, Kazue; Kashihara, Naoki; Makino, Hirofumi; Ota, Zensuke; Ogawa, Norio|
|Publisher:||Journal of Medicine|
This translation by the NDI Foundation is to assist the lay reader. To provide a clear, accessible interpretation of the original article, we eliminated or simplified some technical detail and complicated scientific language. We concentrated our translation on those aspects of the article dealing directly with NDI. The NDI Foundation thanks the researchers for their work toward understanding and more effectively treating this disorder.
© Copyright NDI Foundation 2007 (JC)
They examined diseased kidney tissues from 11 people who had kidney disease, finding that ANP binding sites (the places where the ANP meets the ANP receptor) occurred predominately within the glomeruli -- little filters in the kidney that allow certain dissolved substances from the bloodstream into little tubes (tubules) in the kidney, but prevent cells and large proteins from crossing over. The authors also found that ANP binding occurred, but much less frequently, in kidney tubules. Examination of healthy kidney tissues revealed the same distribution pattern for ANP binding.
Ogura, et al., found fewer glomerular ANP receptors in kidney tissue from patients with kidney dysfunction and hypertension than in tissue from healthy people. There were two exceptions: one case of IgA nephropathy (where the patient had normal kidney function and blood pressure) where the glomerular ANP receptor count was normal, and one case of nephrogenic diabetes insipidus (NDI) where the ANP density was greater than normal. The authors are using their method of in vitro micro-ARG to assess ANP binding in human tissue specimens to further investigate changes in ANP receptor binding.