Rho Inhibits cAMP-Induced Translocation of Aquaporin-2 into the Apical Membrane of Renal Cells
|Title:||Rho Inhibits cAMP-Induced Translocation of Aquaporin-2 into the Apical Membrane of Renal Cells|
|Authors:||Tamma, Grazia; Klussmann, Enno; Maric, Kenan; Aktories, Klaus; Svelto, Maria; Rosenthal, Walter; Valenti, Giovanna|
|Publisher:||American Journal of Physiology: Renal Physiology|
|Date Published:||December 01, 2001|
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This translation by the NDI Foundation is to assist the lay reader. To provide a clear, accessible interpretation of the original article, we eliminated or simplified some technical detail and complicated scientific language. We concentrated our translation on those aspects of the article dealing directly with NDI. The NDI Foundation thanks the researchers for their work toward understanding and more effectively treating this disorder.
© Copyright NDI Foundation 2007 (JC)
The researchers introduced agents that inhibited the function of Rho proteins into laboratory cell cultures rich in AQP2s. They found that when Rho or the Rho kinases were inhibited in the cell cultures, the cultures showed an increase in their ability to pass water. This indicated that the AQP2s were inserted in the cell membranes in the culture. This experiment indicates that Rho inhibits the movement of AQP2 to the cell membrane.
The researchers knew that the protein actin helps prevent the movement of cell particles from the interior of the cell to its membrane. And they knew that proteins of the Rho family help regulate the assembly of actin stress fibers. Their experiment showed that when Rho was inhibited, it caused actin to disassemble, which allowed the AQP2 to travel to the cell membrane.