Amphotericin B Decreases Adenylyl Cyclase Activity and Aquaporin-2 Expression in Rat Kidney
|Title:||Amphotericin B Decreases Adenylyl Cyclase Activity and Aquaporin-2 Expression in Rat Kidney|
|Authors:||Kim, Soo Wan; Yeum, Chung Ho; Kim, Sunmi; Oh, YoonWha; Choi, Ki Chul; Lee, Jongun|
|Publisher:||Journal of Laboratory and Clinical Medicine|
|Date Published:||October 01, 2001|
This translation by the NDI Foundation is to assist the lay reader. To provide a clear, accessible interpretation of the original article, we eliminated or simplified some technical detail and complicated scientific language. We concentrated our translation on those aspects of the article dealing directly with NDI. The NDI Foundation thanks the researchers for their work toward understanding and more effectively treating this disorder.
© Copyright NDI Foundation 2007 (JC)
The researchers injected rats with amphotericin B over a three-week period. As expected, kidney failure occurred. The researchers then examined the kidneys to determine the number of AQP2s (as well as AQP1, AQP3 and Gsa proteins and adenylyl cyclase — other components involved in urine concentration). They found that though the numbers of AQP1 and AQP3 were not significantly affected, the numbers of AQP2 dropped significantly both in the membrane and interior of the collecting duct cells. The number of Gsa protein was decreased, whereas that of adenylyl cyclase were unaltered.
The researchers’ findings suggest that the inability to concentrate urine caused by amphotericin-B may be related to a reduction in the number of AQP2s in the kidney. They also suggest that the G protein may be the site that is primarily injured by amphotericin-B, as indicated by the reduction of Gsa proteins.