Angiotensin II Upregulates the Expression of Vasopressin V2 mRNA in the Inner Medullary Collecting Duct of the Rat
|Title:||Angiotensin II Upregulates the Expression of Vasopressin V2 mRNA in the Inner Medullary Collecting Duct of the Rat|
|Authors:||Wong, Ph.D., Norman L. M.; Tsui, Joseph K. C.|
|Date Published:||March 01, 2003|
This translation by the NDI Foundation is to assist the lay reader. To provide a clear, accessible interpretation of the original article, we eliminated or simplified some technical detail and complicated scientific language. We concentrated our translation on those aspects of the article dealing directly with NDI. The NDI Foundation thanks the researchers for their work toward understanding and more effectively treating this disorder.
© Copyright NDI Foundation 2007 (JC)
To do this, they removed kidneys from rats and created laboratory cell cultures with them. In these they placed varying amounts of angiotensin II for varying amounts of time. The researchers found that modest amounts of angiotensin II incubated with the IMCD cells for 17 hours induced a significant increase in V2R mRNA and a more modest increase in AQP2 mRNA.
When the researchers placed in the cell cultures both angiotensin II and an agent that inhibits angiotensin II, there were much smaller increases in the V2R mRNA and no effect on the AQP2 mRNA. This indicates that it was angiotensin II that caused the increase. To see if protein kinase A (PKA) helped angiotensin II increase V2R mRNA, Wong and Tsui placed a PKA inhibitor in the cell cultures and, upon examination, they found no gain in V2R mRNA. To determine if protein kinase C (PKC) had an effect on the number of V2R mRNA angiotensin II was able to produce, the researchers placed a PKC inhibitor in the cultures. They found that this enabled angiotensin II to greatly increase the number of V2R mRNA.
The researchers then tested the direct effect of PKA and PKC on regulating the expression of V2R mRNA and AQP2 mRNA. Their results suggest that PKA stimulates and PKC suppresses V2R mRNA and AQP2 mRNA in rat kidney IMCD.