Comparative Mapping on the Mouse and Human X Chromosomes of a Human cDNA Clone Encoding the Vasopressin Renal-Type Receptor (AVP2R)
| Title: | Comparative Mapping on the Mouse and Human X Chromosomes of a Human cDNA Clone Encoding the Vasopressin Renal-Type Receptor (AVP2R) |
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| Authors: | Birnbaumer, Mariel; Faust, Cynthia J.; Gonzales, Juanita C.; Seibold, Anita; Herman, Gail E. |
| Publisher: | Genomics |
| Date Published: | February 01, 1993 |
| Reference Number: | 138 |
Mutations in the gene for the human renal-type vasopressin receptor (V2R) have recently been identified in patients with nephrogenic diabetes insipidus (NDI). Both V2R and NDI have been independently mapped to Xq28. Using a combination of genetic and physical mapping, we have localized the murine V2r locus to within 100 kb of L1Cam on the mouse X chromosome in a region syntenic with human Xq28. Based on conserved gene order of mouse and human loci in this region, physical mapping using DNA derived from human lymphoblasts has established that the corresponding human loci V2R and L1CAM are linked within 210 kb. The efficiency and precision of genetic mapping of V2r and other loci in the mouse suggest that it might be easier to map additional human genes in the mouse first and infer the corresponding human location. More precise physical mapping in man could then be performed using pulsed-field gel electrophoresis and/or yeast artificial chromosomes.
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This translation by the NDI Foundation is to assist the lay reader. To provide a clear, accessible interpretation of the original article, we eliminated or simplified some technical detail and complicated scientific language. We concentrated our translation on those aspects of the article dealing directly with NDI. The NDI Foundation thanks the researchers for their work toward understanding and more effectively treating this disorder.
© Copyright NDI Foundation 2007 (JC)
Faust, et al., report on their success in introducing human V2R DNA into mouse X chromosomes and then being able to locate the human V2R gene in the region of the mouse X chromosome that is analogous to the human Xq28 region. The efficiency and precision with which the authors could map the human V2R in mice suggests that it might be easier to map human genes in mouse chromosomes first, on the assumption that the human genes will locate in the same relative area of the mouse gene as they normally would in the human gene. Doing this, researchers could first find the location of the human gene on the mouse gene, infer its location on the human gene and then more precisely map it on the human gene using pulsed-field gel electrophoresis and/or artificial chromosomes.